Simple Plex Human CD21 Cartridge

Name: Simple Plex Human CD21 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000451

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000451

Product Datasheet / COA

Simple Plex Human CD21 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Cerebrospinal Fluid (13 uL)
Sensitivity:	44.3 pg/mL
Assay Range:	91.8 - 140,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human CD21 Cartridge Summary:	Simple Plex assay for the detection of human CD21 in cerebrospinal fluid (CSF),serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD21 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CD21 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CD21.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (pg/mL)	925	41601	883	39241
Standard Deviation	70.6	1969	44.7	1895
CV%	7.6	4.7	5.1	4.8

Linearity

Samples containing and/or spiked with high concentrations of human CD21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CD21 Cartridge

	Human CD21 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD21

CD21, also known as complement receptor 2 (CR2), is a 145 kDa transmembrane protein that contains 15 tandem SCR/SUSHI repeats. CD21 is primarily expressed on B cells, follicular dendritic cells, and T cells. It binds the complement component fragments iC3b, C3d, and C3d,g and forms complexes with CD19 and CD35. CD21 is required for germinal center development, antibody production, and B cell tolerance. It also functions as a cellular uptake receptor for Epstein-Barr virus.

Item	Value
Entrez Gene IDs	1380 (Human); 12902 (Mouse); 289395 (Rat)
Alternate Names	C3DR, C3DRSLEB9, CD21, CD21 antigen, CR2, Complement C3d receptor, EBV receptor, Epstein-Barr virus receptor, complement component (3d/Epstein Barr virus) receptor 2, complement receptor type 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CD21 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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