Simple Plex Human CD25/IL-2R alpha Cartridge

Name: Simple Plex Human CD25/IL-2R alpha Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000239

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000239

Simple Plex Human CD25/IL-2R alpha assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.3 pg/mL
Assay Range:	3.28 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CD25/IL-2R alpha Cartridge Summary:	Simple Plex assay for the detection of human IL-2 R alpha in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD25/IL-2R alpha Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CD25/IL-2R alpha Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-2 R alpha
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	32	1619	27.2	1453
Standard Deviation	1.41	109	2.05	120
CV%	4.4	6.7	7.5	8.2

Linearity

Samples containing and/or spiked with high concentrations of human IL-2 R alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CD25/IL-2R alpha Cartridge

	Human IL-2 R alpha Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD25/IL-2R alpha

CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.

Item	Value
Long Name	Interleukin 2 Receptor alpha
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names	CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL-2Ra, IL2-RA, IL2R, IL2R alpha, IL2RA, TAC antigen, TCGFR, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CD25/IL-2R alpha Cartridge

Product Datasheets

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COA

Protocols & Manuals

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