Simple Plex Human COMP Cartridge

Name: Simple Plex Human COMP Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000756

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000756

Simple Plex Human COMP/Thrombospondin-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.93 pg/mL
Assay Range:	8.44 - 32,196 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human COMP Cartridge Summary:	Simple Plex assay for the detection of human Cartilage Oligomeric Matrix Protein (COMP) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human COMP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human COMP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human COMP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	24
Mean (pg/mL)	234	11010	250	12439
Standard Deviation	17.7	847	32.6	983
CV%	7.6	7.7	13.1	7.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	103	97-111

Linearity

Samples containing and/or spiked with high concentrations of human COMP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human COMP Cartridge

	Human COMP Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: COMP/Thrombospondin-5

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

Item	Value
Long Name	Cartilage Oligomeric Matrix Protein
Entrez Gene IDs	1311 (Human); 12845 (Mouse)
Alternate Names	COMP, EDM1, EPD1, MED, MEDMGC131819, MGC149768, PSACH, THBS5, TSP5, Thrombospondin-5, Thrombospondin5, cartilage oligomeric matrix protein, cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple), cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple), pseudoachondroplasia (epiphyseal dysplasia 1, multiple)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human COMP Cartridge

Product Datasheets

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Protocols & Manuals

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