Simple Plex Human CXCL9/MIG Cartridge Best Seller

Name: Simple Plex Human CXCL9/MIG Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000402

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000402

Simple Plex Human CXCL9/MIG assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3.8 pg/mL
Assay Range:	19.9 - 30,400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CXCL9/MIG Cartridge Summary:	Simple Plex assay for the detection of human MIG in cell culture supernatan (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CXCL9/MIG Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CXCL9/MIG Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MIG.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	185	9368	178	9705
Standard Deviation	15.1	765	18.1	1072
CV%	8.2	8.2	10.2	11.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	100	94-104
Heparin Plasma (n=4)	100	89-109
Serum (n=4)	102	93-111

Linearity

Samples containing and/or spiked with high concentrations of human MIG were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CXCL9/MIG Cartridge

	Human MIG Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Item	Value
Entrez Gene IDs	4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names	C-X-C motif chemokine 9, CMK, CXCL9, Gamma-interferon-induced monokine, Humig, MIG, MIGSmall-inducible cytokine B9, SCYB9Monokine induced by interferon-gamma, chemokine (C-X-C motif) ligand 9, crg-10, monokine induced by gamma interferon

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CXCL9/MIG Cartridge

Product Datasheets

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COA

Protocols & Manuals

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