Simple Plex Human DcR3 Cartridge

Name: Simple Plex Human DcR3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000876

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000876

Simple Plex Human DcR3/TNFRSF6B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.17 pg/mL
Assay Range:	10.49 - 16,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human DcR3 Cartridge Summary:	Simple Plex assay for the detection of human DcR3 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human DcR3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human DcR3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human DcR3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	57	56
Mean (pg/mL)	116	4714	114	5031
Standard Deviation	6.78	375	11.6	473
CV%	5.8	8	10.3	9.4

Linearity

Samples containing and/or spiked with high concentrations of human DcR3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human DcR3 Cartridge

	Human DcR3 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DcR3/TNFRSF6B

Decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%) and Fas (17%), and is expressed in a variety of different tissues and at high levels in many malignant tumors.

Item	Value
Long Name	Decoy Receptor 3
Entrez Gene IDs	8771 (Human); 107126607 (Cynomolgus Monkey)
Alternate Names	DCR3Decoy receptor for Fas ligand, DcR3, Decoy receptor 3, M68, M68DJ583P15.1.1, TNFRSF6B, TR6, TR6DcR3, tumor necrosis factor receptor superfamily member 6B, tumor necrosis factor receptor superfamily, member 6b, decoy

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human DcR3 Cartridge

Product Datasheets

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Protocols & Manuals

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