Simple Plex Human EGF Cartridge

Name: Simple Plex Human EGF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000311

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000311

Product Datasheet / COA

Simple Plex Human EGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL), Urine (10 uL)
Sensitivity:	0.112 pg/mL
Assay Range:	0.2 - 4,680 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Simple Plex Human EGF Cartridge Summary:	Simple Plex assay for the detection of human Epidermal Growth Factor (EGF) in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human EGF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human EGF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human EGF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	9	9
Mean (pg/mL)	30.8	1355	30.1	1467
Standard Deviation	1.19	79.3	1.43	139
CV%	3.9	5.9	4.8	9.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	114	112-117
Platelet-poor EDTA Plasma (n=3)	92	74-103
Platelet-poor Heparin Plasma (n=3)	89	70-103
Serum (n=5)	93	83-107

Linearity

Samples containing and/or spiked with high concentrations of human EGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human EGF Cartridge

	Human EGF Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Item	Value
Long Name	Epidermal Growth Factor
Entrez Gene IDs	1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names	EGF, HOMG4, URG, Urogastrone, beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, pro-epidermal growth factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human EGF Cartridge

Product Datasheets

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COA

Protocols & Manuals

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