Simple Plex Human ENPP-2/Autotaxin Cartridge

Name: Simple Plex Human ENPP-2/Autotaxin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001780

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001780

Simple Plex Human ENPP-2/Autotaxin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	7.55 pg/mL
Assay Range:	31.8 - 121,440 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human ENPP-2/Autotaxin Cartridge Summary:	Simple Plex assay for the detection of human ENPP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human ENPP-2/Autotaxin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human ENPP-2/Autotaxin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ENPP-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	32
Mean (pg/mL)	723	41109	766	36307
Standard Deviation	33.1	2677	77.8	3520
CV%	4.6	6.5	10.2	9.7

Linearity

Samples containing and/or spiked with high concentrations of human ENPP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human ENPP-2/Autotaxin Cartridge

	Human ENPP-2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ENPP-2/Autotaxin

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

Item	Value
Long Name	Ectonucleotide Pyrophosphatase/Phosphodiesterase 2
Entrez Gene IDs	5168 (Human); 18606 (Mouse); 84050 (Rat)
Alternate Names	ATX, ATX-X, ATXFLJ26803, Autotaxin, E-NPP 2, EC 3.1.4.39, ENPP-2, ENPP2, Extracellular lysophospholipase D, LysoPLD, Lysophosphatidic Acid, NPP2, PD-IALPHA, PDNP2, PDNP2NPP2, autotaxin-t, ectonucleotide pyrophosphatase/phosphodiesterase 2, ectonucleotide pyrophosphatase/phosphodiesterase family member 2, phosphodiesterase I/nucleotide pyrophosphatase 2, plasma lysophospholipase D

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human ENPP-2/Autotaxin Cartridge

Product Datasheets

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Protocols & Manuals

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