Simple Plex Human Ferritin Cartridge

Name: Simple Plex Human Ferritin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001044

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001044

Simple Plex Human Ferritin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Cerebrospinal Fluid (5 uL)
Sensitivity:	2.9 pg/mL
Assay Range:	16.6 - 63,240 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human Ferritin Cartridge Summary:	Simple Plex assay for the detection of human Ferritin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Ferritin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Ferritin Cartridge

Reactivity	Human
Specificity	Natural and recombinant human Ferritin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	32
Mean (µL)	355	20037	362	20915
Standard Deviation	12.3	1650	28.8	2318
CV%	3.5	8.2	8	11.1

Linearity

Samples containing and/or spiked with high concentrations of human Ferritin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Ferritin Cartridge

	Human Ferritin Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Ferritin

Item	Value
Entrez Gene IDs	2495 (Human)
Alternate Names	Cell proliferation-inducing gene 15 protein, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, Ferritin, Ferritin H subunit, PIG15, PLIF, apoferritin, ferritin heavy chain, ferritin, heavy polypeptide 1, placenta immunoregulatory factor, proliferation-inducing protein 15

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Ferritin Cartridge

Product Datasheets

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Protocols & Manuals

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