Simple Plex Human Granzyme B Cartridge

Name: Simple Plex Human Granzyme B Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000496

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000496

Simple Plex Human Granzyme B assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.385 pg/mL
Assay Range:	1.31 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Granzyme B Cartridge Summary:	Simple Plex assay for the detection of human Granzyme B in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Granzyme B Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Granzyme B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Granzyme B
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	20	19
Mean (pg/mL)	28	1357	28.7	1377
Standard Deviation	1.26	47.1	2.87	143
CV%	4.5	3.5	10	10.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	103	91-117
Heparin Plasma (n=4)	95	86-107
Serum (n=4)	105	91-119

Linearity

Samples containing and/or spiked with high concentra- tions of human Granyme B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Granzyme B Cartridge

	Human Granzyme B Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Item	Value
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
Alternate Names	C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, GRB, GZMB, Granzyme B, Granzyme-2, GranzymeB, GrzB, HLP, SECT

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Granzyme B Cartridge

Product Datasheets

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Protocols & Manuals

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