Simple Plex Human Growth Hormone Cartridge

Name: Simple Plex Human Growth Hormone Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000492

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000492

Simple Plex Human Growth Hormone assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.67 pg/mL
Assay Range:	1.74 - 16,610 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Growth Hormone Cartridge Summary:	Simple Plex assay for the detection of human GH in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Growth Hormone Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Growth Hormone Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human GH.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	32
Mean (pg/mL)	93.8	4195	87.6	4425
Standard Deviation	5.86	334	6.63	397
CV%	6.3	8	7.6	9

Linearity

Samples containing and/or spiked with high concentrations of human GH were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Growth Hormone Cartridge

	Human Growth Hormone Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.

Item	Value
Entrez Gene IDs	2688 (Human)
Alternate Names	GH-N, GH-Nsomatotropin, GH1, GHB5, GHIGHD1B, GHN, GHNGrowth hormone, Growth Hormone, IGHD1A, IGHD2, Somatotropin, growth hormone 1Pituitary growth hormone, hGH-N

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Growth Hormone Cartridge

Product Datasheets

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Protocols & Manuals

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