Simple Plex Human Hepcidin Cartridge

Name: Simple Plex Human Hepcidin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000984

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000984

Simple Plex Human Hepcidin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	4.81 pg/mL
Assay Range:	9.7 - 37,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Hepcidin Cartridge Summary:	Simple Plex assay for the detection of human Hepcidin in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Hepcidin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Hepcidin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Hepcidin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	48	50
Mean (pg/mL)	209	10447	218	12032
Standard Deviation	10.3	789	27.5	1624
CV%	4.9	7.6	12.6	13.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	109	106-112
Heparin Plasma (n=4)	100	92-113
Serum (n=4)	109	106-117

Linearity

Samples containing and/or spiked with high concentrations of human Hepcidin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Hepcidin Cartridge

	Human Hepcidiin Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Hepcidin

Item	Value
Long Name	Hepcidin Antimicrobial Peptide
Entrez Gene IDs	57817 (Human); 84506 (Mouse); 84604 (Rat)
Alternate Names	HAMP, HEPC, HEPCPutative liver tumor regressor, HFE2B, HFE2Bhepcidin, Hepcidin, LEAP-1, LEAP1, LEAP1PLTR, Liver-expressed antimicrobial peptide 1, PLTR, hepcidin antimicrobial peptide

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Hepcidin Cartridge

Product Datasheets

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COA

Protocols & Manuals

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