Simple Plex Human HGF Cartridge

Name: Simple Plex Human HGF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000143

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000143

Product Datasheet / COA

Simple Plex Human HGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3.77 pg/mL
Assay Range:	9.94 - 15,160 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human HGF Cartridge Summary:	Simple Plex assay for the detection of human HGF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human HGF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human HGF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human HGF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	9
Mean (pg/mL)	87.1	4038	89.3	4416
Standard Deviation	5.03	339	3.24	316
CV%	5.8	8.4	3.6	7.2

Linearity

Samples containing and/or spiked with high concentrations of human HGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human HGF Cartridge

	Human HGF Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Item	Value
Long Name	Hepatocyte Growth Factor
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names	DFNB39, EC 3.4.21, EC 3.4.21.7, F-TCF, HGF, HGFB, HPTA, HPTAhepatocyte growth factor, Hepatopoeitin-A, Hepatopoietin A, SF, SFhepatopoeitin-A, Scatter factor, deafness, autosomal recessive 39, fibroblast-derived tumor cytotoxic factor, hepatocyte growth factor (hepapoietin A; scatter factor), lung fibroblast-derived mitogen

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human HGF Cartridge

Product Datasheets

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COA

Protocols & Manuals

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