Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge

Name: Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-003329

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003329

Simple Plex Human IGFBP-rp1/IGFBP-7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity:	0.64 pg/mL
Assay Range:	5.24 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge Summary:	Simple Plex assay for the detection of human IGFBP-rp1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IGFBP-rp1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	44	44
Mean (pg/mL)	50.9	2615	54.9	2862
Standard Deviation	4.47	169	5.79	307
CV%	8.8	6.5	10.5	10.7

Linearity

Samples containing and/or spiked with high concentrations of human IGFBP-rp1 were serially diluted with Sample Diluent to produce samples within the

dynamic range of the assay.

Human IGFBP-rp1/IGFBP-7 Ella Assay Linearity

Scientific Data Examples for Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge

	Human IGFBP-7 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-rp1/IGFBP-7

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Item	Value
Long Name	Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs	3490 (Human); 29817 (Mouse)
Alternate Names	FSTL2, IBP-7, IGFBP-7, IGFBP-7PGI2-stimulating factor, IGFBP-7v, IGFBP-rp1, IGFBP7, IGFBPrp1, MAC25 protein, MAC25IGF-binding protein 7, Mac25, PSF, PSFAGM, Prostacyclin-stimulating factor, TAF, Tumor-derived adhesion factor, angiomodulin, insulin-like growth factor binding protein 7, insulin-like growth factor-binding protein 7

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human IGFBP-rp1/IGFBP-7 Cartridge

Product Datasheets

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Protocols & Manuals

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