Simple Plex Human IL-18 BPa Cartridge

Name: Simple Plex Human IL-18 BPa Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000502

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000502

Simple Plex Human IL-18 BPa assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bronchoalveolar Lavage Fluid (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.881 pg/mL
Assay Range:	2.84 - 4,336 pg/mL (Bronchoalveolar Lavage Fluid, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IL-18 BPa Cartridge Summary:	Simple Plex assay for the detection of human IL-18 BPa in serum, plasma (EDTA/Heparin), and bronchoalveolar lavage fluid (BALF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-18 BPa Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IL-18 BPa Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-18 BPa.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Linearity

Samples containing and/or spiked with high concentrations of human IL-18 BPa were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IL-18 BPa Cartridge

	Human IL-18 BPa Ella Assay Standard Curve
	Human IL-18 BPa Ella Assay Linearity

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18 BPa

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

Item	Value
Long Name	Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs	10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names	IL-18 BPa, IL-18BP, IL18 BPa, IL18BP, IL18BPa, MC51L-53L-54L homolog gene product, interleukin 18 binding protein, interleukin-18-binding protein, tadekinig-alfa

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human IL-18 BPa Cartridge

Product Datasheets

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COA

Protocols & Manuals

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