Simple Plex Human IL-5 Cartridge

Name: Simple Plex Human IL-5 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000229

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000229

Simple Plex Cartridge Kit for quantitation of IL-5. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.02 pg/mL
Assay Range:	0.13 - 1,248 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IL-5 Cartridge Summary:	Simple Plex assay for the detection of human IL-5 in cell culture superntant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-5 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IL-5 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-5.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	10
Mean (pg/mL)	22.5	999	19	886
Standard Deviation	1.69	54.9	1.9	73.3
CV%	7.5	5.5	10.1	8.3

Recovery

Recovery at three different spiked concentrations withinthe range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	87-102
Heparin Plasma (n=4)	95	85-101
Serum (n=4)	99	90-105

Linearity

Samples containing and/or spiked with high concentrations of human IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IL-5 Cartridge

	Human IL-5 Ella Assay Standard Curve

Preparation and Storage

Shipping	Lyophilized product is shipped at ambient temperature. Upon receipt, store immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Item	Value
Long Name	Interleukin 5
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names	B-cell differentiation factor I, BCDF mu, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL-5, IL-5T-cell replacing factor, IL5, TRF, TRFB cell differentiation factor I, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human IL-5 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol