Simple Plex Human IL-7 Cartridge

Name: Simple Plex Human IL-7 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000506

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000506

Simple Plex Cartridge Kit for quantitation of IL-7. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.08 pg/mL
Assay Range:	0.57 - 2,170 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IL-7 Cartridge Summary:	Simple Plex assay for the detection of human IL-7 in cell culture supernatan (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-7 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IL-7 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IL-7.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	1
n	16	16	12	12
Mean (pg/mL)	11.9	622	12	628
Standard Deviation	0.454	35.5	0.58	28.7
CV%	3.8	5.7	4.8	4.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	93	91-98
EDTA Plasma (n=4)	95	90-102
Heparin Plasma (n=4)	76	70-84
Serum (n=4)	96	90-101

Linearity

Samples containing and/or spiked with high concentrations of human IL-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human IL-7 Cartridge

	Human IL-7 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Item	Value
Long Name	Interleukin 7
Entrez Gene IDs	3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names	IL-7, IL-7interleukin-7, IL7, Lymphopoietin-1, PBGF, interleukin 7

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human IL-7 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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