Simple Plex Human LBP Cartridge

Name: Simple Plex Human LBP Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000508

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000508

Product Datasheet / COA

Simple Plex Human LBP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	6.88 pg/mL
Assay Range:	98.3 - 150,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human LBP Cartridge Summary:	Simple Plex assay for the detection of human LBP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human LBP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human LBP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human LBP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	37
Mean (pg/mL)	1025	51532	1036	51807
Standard Deviation	71.4	3306	116	4349
CV%	7	6.4	11.1	8.4

Linearity

Samples containing and/or spiked with high concentrations of human LBP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human LBP Cartridge

	Human LBP Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins that includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipidA portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyzes the binding of LPS monomers to CD14, and promotes LPS-induced immune response.

Item	Value
Long Name	Lipopolysaccharide-binding Protein
Entrez Gene IDs	3929 (Human); 16803 (Mouse)
Alternate Names	LBP, LPS-binding protein, MGC22233, lipopolysaccharide binding protein, lipopolysaccharide-binding protein

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human LBP Cartridge

Product Datasheets

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COA

Protocols & Manuals

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