Simple Plex Human M-CSF Cartridge

Name: Simple Plex Human M-CSF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000403

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000403

Product Datasheet / COA

Simple Plex Human M-CSF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bronchoalveolar Lavage Fluid (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	1.02 pg/mL
Assay Range:	3.11 - 11,880 pg/mL (Bronchoalveolar Lavage Fluid, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human M-CSF Cartridge Summary:	Simple Plex assay for the detection of human M-CSF in serum, plasma (EDTA/Heparin), and bronchoalveolar lavage fluid (BALF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human M-CSF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human M-CSF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human M-CSF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	91	82-101
Heparin Plasma (n=4)	90	84-96
Serum (n=4)	90	88-95

Linearity

Samples containing and/or spiked with high concentrations of human M-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human M-CSF Cartridge

	Human M-CSF Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Item	Value
Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Alternate Names	CSF-1, CSF1, Lanimostim, M-CSF, MCSF, MCSFlanimostim, MGC31930, colony stimulating factor 1 (macrophage), macrophage colony stimulating factor, macrophage colony-stimulating factor 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human M-CSF Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol