Simple Plex Human Neutrophil Elatase/ELA2 Cartridge

Name: Simple Plex Human Neutrophil Elatase/ELA2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-011934

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011934

COA

Simple Plex Human Neutrophil Elatase/ELA2 assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD06, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	3.97 pg/mL
Assay Range:	16.38 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Neutrophil Elatase/ELA2 Cartridge Summary:	Simple Plex assay for the detection of human Human Neutrophil Elastase (ELA2) in cell culture supernates (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Neutrophil Elatase/ELA2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Neutrophil Elatase/ELA2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ELA2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	78	53
Mean (pg/mL)	63.9	4161	68.5	3515
Standard Deviation	2.39	203	8.1	325
CV%	3.7	4.9	11.9	9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	110	109-110

Linearity

Samples containing and/or spiked with high concentrations of human ELA2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Neutrophil Elatase/ELA2 Cartridge

	Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Item	Value
Entrez Gene IDs	1991 (Human); 50701 (Mouse)
Alternate Names	Bone marrow serine protease, EC 3.4.21, EC 3.4.21.37, ELA2, ELA2granulocyte-derived elastase, ELANE, Elastase-2, GE, HLEelastase-2, HNE, Human leukocyte elastase, Leukocyte Elastase, Medullasin, NE, Neutrophil Elastase, PMN elastase, PMN-E, SCN1, elastase 2, neutrophil, elastase, neutrophil expressed, polymorphonuclear elastase

Compatible Instruments

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