Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Name: Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000321

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000321

Simple Plex Human Proprotein Convertase 9/PCSK9 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	4.32 pg/mL
Assay Range:	14.9 - 56,970 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge Summary:	Simple Plex assay for the detection of human PCSK9 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human PCSK9.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	22
Mean (pg/mL)	296	17116	292	17472
Standard Deviation	19.6	842	18.3	1101
CV%	6.6	4.9	6.3	6.3

Linearity

Samples containing and/or spiked with high concentrations of human PCSK9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

	Human PCSK9 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Proprotein Convertase 9/PCSK9

This family of serine proteases consists of furin and proprotein convertases encoded by PCSK genes. They are important in a variety of processes. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. Mutations in the PC1/PCSK1 gene are associated with obesity and impaired prohormone processing.

Item	Value
Entrez Gene IDs	255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names	EC 3.4.21, EC 3.4.21.111, FH3, FH3neural apoptosis regulated convertase 1, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC-1convertase subtilisin/kexin type 9 preproprotein, NARC1, NARC1EC 3.4.21.-, Neural apoptosis-regulated convertase 1, PC9, PCSK9, Proprotein Convertase 9, Subtilisin/kexin-like protease PC9, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Product Datasheets

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Protocols & Manuals

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