Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Name: Simple Plex Human Pentraxin 3/TSG-14 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000537

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000537

Simple Plex Human Pentraxin 3/TSG-14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)
Sensitivity:	2.26 pg/mL
Assay Range:	5.2 - 32,530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Simple Plex Human Pentraxin 3/TSG-14 Cartridge Summary:	Simple Plex assay for the detection of human Pentraxin 3 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and saliva.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Pentraxin 3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	17
Mean (pg/mL)	206	9568	211	9837
Standard Deviation	10.1	46	11.7	552
CV%	4.9	4.8	5.5	5.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	105	103-110
EDTA Plasma (n=4)	98	92-109
Heparin Plasma (n=4)	102	98-109

Linearity

Samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

	Human Pentraxin 3/TSG-14 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Item	Value
Long Name	Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs	5806 (Human); 19288 (Mouse)
Alternate Names	PTX3, Pentaxin-related protein PTX3, Pentraxin 3, TNF alpha-induced protein 5, TNFAIP5, TSG-14, TSG-14pentaxin-related gene, rapidly induced by IL-1 beta, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, alpha-induced protein 5, pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, pentraxin 3, long, pentraxin-3, pentraxin-related gene, rapidly induced by IL-1 beta, pentraxin-related protein PTX3, tumor necrosis factor, alpha-induced protein 5, tumor necrosis factor-inducible protein TSG-14

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

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Protocols & Manuals

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