Simple Plex Human SP-D Cartridge

Name: Simple Plex Human SP-D Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000529

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000529

Citations (1)

Product Datasheet / COA

Simple Plex Human SP-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	19.1 pg/mL
Assay Range:	154 - 93,810 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human SP-D Cartridge Summary:	Simple Plex assay for the detection of human Surfactant Protein D (SP-D) cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human SP-D Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human SP-D Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human SP-D.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	25
Mean (pg/mL)	628	26121	532	26305
Standard Deviation	48.7	1603	61.3	3115
CV%	7.8	6.1	11.5	11.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	105	94-125

Linearity

Samples containing and/or spiked with high concentrations of human SP-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human SP-D Cartridge

	Human SP-D Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Item	Value
Long Name	Surfactant Pulmonary Associated Protein D
Entrez Gene IDs	6441 (Human); 20390 (Mouse)
Alternate Names	COLEC7collectin-7, Collectin 7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, SFTP4, SFTP4pulmonary surfactant-associated protein D, SFTPD, SP-D, SP-Dpulmonary surfactant apoprotein, SPD, surfactant protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary 4

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human SP-D Cartridge

Product Datasheets

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COA

Protocols & Manuals

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