Simple Plex Human Syndecan-4 Assay Cartridge

Name: Simple Plex Human Syndecan-4 Assay Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-009775

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-009775

Simple Plex Human Syndecan-4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	1.28 pg/mL
Assay Range:	5.05 - 7,710 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human Syndecan-4 Assay Cartridge Summary:	Simple Plex assay for the detection of human Syndecan-4 in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Syndecan-4 Assay Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Syndecan-4 Assay Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Syndecan-4.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	46
Mean (pg/mL)	55.4	2887	59	2958
Standard Deviation	4.55	150	5.7	259
CV%	8.2	5.2	9.7	8.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	99-105
Cerebrospinal Fluid (n=3)	109	104-115

Linearity

Samples containing and/or spiked with high concentrations of human Syndecan-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Syndecan-4 Assay Cartridge

	Human Syndecan-4 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Syndecan-4

Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. Syndecans are present as homodimers or multimers, and are often expressed in developmental and cell type-specific patterns. They have many biological roles including regulating cell growth, differentiation, and adhesion.

Item	Value
Entrez Gene IDs	6385 (Human); 20971 (Mouse)
Alternate Names	Amphiglycan, MGC22217, Ryudocan, Ryudocan core protein, SDC4, SYND4ryudocan amphiglycan, Syndecan-4, Syndecan4, syndecan 4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Syndecan-4 Assay Cartridge

Product Datasheets

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Protocols & Manuals

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