Simple Plex Human TACI/TNFRSF13B Cartridge

Name: Simple Plex Human TACI/TNFRSF13B Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000381

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000381

Simple Plex Human TACI/TNFRSF13B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.534 pg/mL
Assay Range:	2.52 - 24,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human TACI/TNFRSF13B Cartridge Summary:	Simple Plex assay for the detection of human TACI in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TACI/TNFRSF13B Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TACI/TNFRSF13B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TACI.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (µL)	32.7	2845	33.4	3209
Standard Deviation	0.912	90.6	2.56	255
CV%	2.8	3.2	7.7	7.9

Linearity

Samples containing and/or spiked with high concentrations of human TACI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human TACI/TNFRSF13B Cartridge

	Human TACI/TNFRSF13B Ella Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily (TNFRSF). Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA both bind APRIL and BLyS/BAFF, members of the TNF superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Human and mouse TACI share 54% amino acid sequence identity.

Item	Value
Long Name	Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Entrez Gene IDs	23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)
Alternate Names	CD267, CD267 antigen, CVID, CVID2, FLJ39942, MGC133214, TACI, TACIMGC39952, TNFRSF13B, TNFRSF14B, Transmembrane activator and CAML interactor, tumor necrosis factor receptor 13B, tumor necrosis factor receptor superfamily member 13B, tumor necrosis factor receptor superfamily, member 13B

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TACI/TNFRSF13B Cartridge

Product Datasheets

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COA

Protocols & Manuals

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