Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Name: Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002900

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002900

Simple Plex TIM-1/KIM-1/HAVCR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)
Sensitivity:	0.55 pg/mL
Assay Range:	3.41 - 5,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge Summary:	Simple Plex assay for the detection of human TIM-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TIM-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	56	59
Mean (pg/mL)	36.5	1941	37.2	1853
Standard Deviation	3.35	93.5	4.49	185
CV%	9.2	4.8	12.1	10

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	96	91-102
Heparin Plasma (n=4)	93	89-98
Serum (n=4)	104	97-108

Linearity

Samples containing and/or spiked with high concentrations of human TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

	Human TIM-1/KIM-1/HAVCR Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Item	Value
Long Name	T Cell Immunoglobulin Mucin-1
Entrez Gene IDs	26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names	CD365, HAVCR-1, HAVCR1, HAVCRT cell immunoglobin domain and mucin domain protein 1, KIM-1, KIM1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, TIM-1, TIM-1TIM, TIM1, TIM1TIMD-1, TIMD1T-cell membrane protein 1, hepatitis A virus cellular receptor 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Product Datasheets

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Protocols & Manuals

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