Simple Plex Human TNF RII/TNFRSF1B Cartridge

Name: Simple Plex Human TNF RII/TNFRSF1B Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000535

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000535

Simple Plex Human TNF RII/TNFRSF1B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity:	0.238 pg/mL
Assay Range:	1.35 - 2,060 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human TNF RII/TNFRSF1B Cartridge Summary:	Simple Plex assay is for the detection of human Tumor Necrosis Factor Receptor II (TNF RII) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TNF RII/TNFRSF1B Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TNF RII/TNFRSF1B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TNF RII.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision	Inter-Assay Precision
Sample	1	1	2	2
n	16	28	16	24
Mean (pg/mL)	13.8	12.4	622	588
Standard Deviation	0.613	1.09	33	39
CV%	4.4	8.8	5.3	6.6

Linearity

Samples containing and/or spiked with high concentrations of human TNF RII were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human TNF RII/TNFRSF1B Cartridge

	Human TNF RII/TNFRSF1B Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RII/TNFRSF1B

TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

Item	Value
Long Name	Tumor Necrosis Factor Receptor II
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names	CD120b, CD120b antigen, Etanercept, TNF RII, TNF-R-II, TNF-R2, TNF-R75, TNF-RII, TNFBRp80 TNF-alpha receptor, TNFR-II, TNFR2TNFR1B, TNFR80, TNFRII, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75 TNF receptor, p75TBPII, p75TNFR, soluble TNFR1B variant 1, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, tumor necrosis factor receptor superfamily member 1B, tumor necrosis factor receptor superfamily, member 1B

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TNF RII/TNFRSF1B Cartridge

Product Datasheets

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Protocols & Manuals

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