Simple Plex Human Total Tau Cartridge

Name: Simple Plex Human Total Tau Cartridge
Brand: ProteinSimple
SKU: SPCKC-PS-009562

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-009562

Simple Plex Human Total Tau assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.13 pg/mL
Assay Range:	0.84 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human Total Tau Cartridge Summary:	Simple Plex assay for the detection of human Total Tau in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Total Tau Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Total Tau Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant Total Tau.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	35
Mean (pg/mL)	70.7	2893	72.4	2818
Standard Deviation	5.55	260	5.7	204
CV%	7.9	9	7.9	7.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	99-108
EDTA Plasma (n=4)	105	102-109
Heparin Plasma (n=4)	104	101-108
Serum (n=4)	103	93-111

Linearity

Scientific Data Examples for Simple Plex Human Total Tau Cartridge

	Human Total Tau Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tau

Tau protein serves as a bridge from microtubules to the plasma membrane to facilitate formation and stabilization of the microtubule network. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kDa in size, but multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat.

Item	Value
Long Name	Microtubule-Associated Protein Tau
Entrez Gene IDs	4137 (Human); 17762 (Mouse); 29477 (Rat)
Alternate Names	DDPAC, FLJ31424, FTDP-17, G protein beta1/gamma2 subunit-interacting factor 1, MAPT, MGC138549, MSTD, MSTDMAPTL, MTBT1, MTBT1Neurofibrillary tangle protein, MTBT2, Neurofibrillary tangle protein, PHF-tau, PPND, TAUPaired helical filament-tau, Tau, microtubule-associated protein tau

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Total Tau Cartridge

Product Datasheets

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COA

Protocols & Manuals

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