Simple Plex Human VEGF-D Cartridge

Name: Simple Plex Human VEGF-D Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-009111

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-009111

Simple Plex Human VEGF-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	4.55 pg/mL
Assay Range:	40.96 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human VEGF-D Cartridge Summary:	Simple Plex assay for the detection of human VEGF-D in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human VEGF-D Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human VEGF-D Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human VEGF-D.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	43	43
Mean (pg/mL)	93.3	4309	88.5	4192
Standard Deviation	3.93	164	8.12	399
CV%	4.2	3.8	9.2	9.5

Recovery

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	95	92-99
EDTA Plasma (n=4)	90	81-101
Heparin Plasma (n=4)	94	90-101
Serum (n=4)	88	77-96

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human VEGF-D Cartridge

	Human VEGF-D Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-D

Vascular endothelial growth factor D (VEGF-D) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-D containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-D is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium. While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, while VEGF-D is most active in neonatal lymphatic maturation and bone growth.

Item	Value
Long Name	Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
Entrez Gene IDs	2277 (Human); 14205 (Mouse)
Alternate Names	FIGF, VEGF-D, VEGF-DVEGFDc-Fos-induced growth factor, VEGFD, c-fos induced growth factor (vascular endothelial growth factor D), vascular endothelial growth factor D

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human VEGF-D Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol