Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

Name: Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-001192

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-001192

Simple Plex Mouse CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL)
Sensitivity:	3.32 pg/mL
Assay Range:	26 - 15,860 pg/mL (Serum)
Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge Summary:	Simple Plex assay for the detection of mouse SDF-1 alpha in serum.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse SDF-1 alpha.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	22
Mean (pg/mL)	96.1	4737	91.9	4984
Standard Deviation	9.57	389	7.36	350
CV%	10	8.2	8	7

Linearity

Samples containing and/or spiked with high concentrations mouse SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

	Mouse CXCL12/SDF-1 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Item	Value
Entrez Gene IDs	6387 (Human); 20315 (Mouse); 493806 (Feline)
Alternate Names	CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1, SDF1 alpha, SDF1B, SDF1a, TLSF, TPAR1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

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Protocols & Manuals

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