Simple Plex Mouse GDF-15 Cartridge

Name: Simple Plex Mouse GDF-15 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008561

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008561

Simple Plex Mouse GDF-15 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)
Sensitivity:	0.1 pg/mL
Assay Range:	0.32 - 1,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Mouse GDF-15 Cartridge Summary:	Simple Plex assay for the detection of mouse Growth Differentiation Factor 15 (GDF-15) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse GDF-15 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse GDF-15 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	60	60
Mean (pg/mL)	8.73	416	7.83	401
Standard Deviation	0.356	26.2	0.805	29.6
CV%	4.1	6.3	10.3	7.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	104-105

Linearity

Samples containing high concentrations of mouse GDF-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse GDF-15 Cartridge

	Mouse GDF-15 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-15

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Item	Value
Long Name	Growth Differentiation Factor 15
Entrez Gene IDs	9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names	GDF-15, GDF15, MIC-1, MIC-1NSAID-activated gene 1 protein, MIC1Prostate differentiation factor, Macrophage inhibitory cytokine 1, NAG-1, NAG-1NSAID-regulated gene 1 protein, NSAID (nonsteroidal inflammatory drug)-activated protein 1, PDF, PDFGDF-15, PLAB, PLABNRG-1, PTGF-beta, PTGFBPTGF-beta, Placental TGF-beta, Placental bone morphogenetic protein, growth differentiation factor 15, growth/differentiation factor 15

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse GDF-15 Cartridge

Product Datasheets

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Protocols & Manuals

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