Simple Plex Mouse/Rat Cystatin C Cartridge

Name: Simple Plex Mouse/Rat Cystatin C Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-005830

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-005830

Simple Plex Cartridge Kit for quantitation of Cystatin C. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Serum (25 nL), Rat Serum (25 nL), Mouse EDTA Plasma (25 nL), Rat EDTA Plasma (25 nL), Urine (25 nL)
Sensitivity:	0.27 pg/mL
Assay Range:	0.94 - 3,590 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine)
Simple Plex Mouse/Rat Cystatin C Cartridge Summary:	Simple Plex assay for the detection of mouse/rat Cystatin C in serum, EDTA plasma, and urine

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat Cystatin C Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat Cystatin C Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse/rat Cystatin C.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	19
Mean (pg/mL)	20.7	991	18.7	885
Standard Deviation	1.26	90.7	1.4	88.9
CV%	6.1	9.1	7.5	10

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Cystatin C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Rat Cystatin C Simple Plex Assay Linearity

Scientific Data Examples for Simple Plex Mouse/Rat Cystatin C Cartridge

	Mouse/Rat Cystatin C Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Item	Value
Entrez Gene IDs	1471 (Human); 13010 (Mouse); 25307 (Rat)
Alternate Names	ARMD11, CST3, Cystatin C, Gamma-trace, MGC117328, Neuroendocrine basic polypeptide, Post-gamma-globulin, bA218C14.4 (cystatin C), cystatin 3, cystatin C (amyloid angiopathy and cerebral hemorrhage), cystatin-3, cystatin-C

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat Cystatin C Cartridge

Product Datasheets

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Protocols & Manuals

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