Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Name: Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008305

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008305

Simple Plex Mouse/Rat VCAM-1/CD106 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	1.59 pg/mL
Assay Range:	7.97 - 30,372 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge Summary:	Simple Plex assay for the detection of mouse and rat VCAM-1 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Rat
Specificity	natural mouse and rat and recombinant mouse VCAM-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	44	43
Mean (pg/mL)	197	9548	198	10414
Standard Deviation	8.38	546	21.2	1216
CV%	4.2	5.7	10.7	11.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	105	103-107

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat VCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

	Mouse/Rat VCAM-1/CD106 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background:

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.

Item	Value

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Product Datasheets

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Protocols & Manuals

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