Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge

Name: Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-009156

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009156

Product Datasheet / COA

Simple Plex Mouse ST2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	2.35 pg/mL
Assay Range:	15.73 - 24,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge Summary:	Simple Plex assay for the detection of mouse ST2/IL-33R/IL-1R4 in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse ST2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	36	40
Mean (pg/mL)	197	9544	186	9232
Standard Deviation	16.8	805	21.6	647
CV%	8.5	8.4	11.6	7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	109	102-115

Linearity

Samples containing and/or spiked with high concentrations of mouse ST2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge

	Mouse ST2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33R

ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

Item	Value
Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Alternate Names	DER4, DER4ST2growth stimulation-expressed, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, MGC32623, Protein ST2, ST2, ST2L, ST2V, T1, T1homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, interleukin-1 receptor-like 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Mouse ST2/IL-33R/IL-1R4 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol