Simple Plex Rat GDF-8/Myostatin Cartridge

Name: Simple Plex Rat GDF-8/Myostatin Cartridge
Brand: ProteinSimple
SKU: SPCKB-RP-012980

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-RP-012980

Product Datasheet / COA

Simple Plex Rat GDF-8 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD63, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.41 pg/mL
Assay Range:	1.9 - 18,091 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Rat GDF-8/Myostatin Cartridge Summary:	Simple Plex assay for the detection of rat Growth Differentiation Factor 8 (GDF-8) in serum and plasma (EDTA/Heparin) in Sample Diluent SD63.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Rat GDF-8/Myostatin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Rat GDF-8/Myostatin Cartridge

Reactivity	Rat
Specificity	Natural and recombinant GDF-8.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	68	65
Mean (pg/mL)	224	9507	140	6255
Standard Deviation	6.36	460	14.9	574
CV%	2.8	4.8	10.7	9

Linearity

Samples containing and/or spiked with high concentrations of GDF-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Rat GDF-8/Myostatin Cartridge

	Simple Plex Rat GDF-8 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-8/Myostatin

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Item	Value
Long Name	Growth Differentiation Factor 8
Entrez Gene IDs	2660 (Human); 17700 (Mouse)
Alternate Names	GDF-8, GDF8, GDF8growth differentiation factor 8, MSLHP, MSTN, Myostatin, growth/differentiation factor 8

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.