Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Name: Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge
Brand: ProteinSimple
SKU: SPCKB-RP-000548

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-RP-000548

Simple Plex Rat TIM-1/KIM-1/HAVCR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Rat Serum (10 uL), Rat EDTA Plasma (10 uL), Rat Urine (10 uL)
Sensitivity:	2.89 pg/mL
Assay Range:	7.06 - 10,770 pg/mL (Cell Culture Supernates, Rat Serum, Rat EDTA Plasma, Rat Urine)
Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge Summary:	Simple Plex assay for the detection of rat T Cell Immunoglobulin Mucin (TIM-1) in serum, EDTA plasma, and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Rat
Specificity	Natural and recombinant rat TIM-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Sample Type	Average % Recovery	Range %
Rat EDTA Plasma (n=4)	102	94-113
Rat Serum (n=4)	103	94-111
Rat Urine (n=4)	110	103-115

Linearity

Samples containing and/or spiked with high concentrations of rat TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

	Rat TIM-1/KIM-1/HAVCR Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Item	Value
Long Name	T Cell Immunoglobulin Mucin-1
Entrez Gene IDs	26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names	CD365, HAVCR-1, HAVCR1, HAVCRT cell immunoglobin domain and mucin domain protein 1, KIM-1, KIM1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, TIM-1, TIM-1TIM, TIM1, TIM1TIMD-1, TIMD1T-cell membrane protein 1, hepatitis A virus cellular receptor 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Product Datasheets

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Protocols & Manuals

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