Lysis Kit - RIPA Buffer for Charge Assays
ProteinSimple, part of Bio-Techne | Catalog # CBS401
Compatible with Peggy Sue or NanoPro 1000 systems
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Key Product Details
- RIPA Buffer contains, 20 mM HEPES (pH 7.5), 150 mM NaCl, 1% NP-40, 0.25% Sodium Deoxycholate, 10% Glycerol, 1X DMSO and aqueous inhibitor mix.
- Aqueous Protease Inhibitor contains 40mM NaF, 12 mM β-glycerophosphate, 1 mM Sodium Orthovanadate, 0.5mM EDTA, 2.5 mM EGTA
- DMSO Inhibitor Mix contains 1mM AEBSF, 5µg/ml Aprotinin, 50 µM Bestatin, 5µg/ml E-64 Protease Inhibitor, 10 µg/ml Leupeptin, 7µg/mL Pepstatin A, Phosphatase inhibitor cocktail (with effective concentrations of Cantharidin, Bromotetramisole and Microcystin LR)
Compatible Instruments
Peggy Sue - Automated Western Blot System | ProteinSimple
