1400W dihydrochloride

Name: 1400W dihydrochloride
Brand: Tocris Bioscience
SKU: 1415
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1415

(3)

Citations (20)

COA / SDS

Potent, highly selective iNOS inhibitor

Key Product Details

Description:	Potent, highly selective iNOS inhibitor
Chemical Name:	N-[[3-(Aminomethyl)phenyl]methyl]-ethanimidamide dihydrochloride
Purity:	≥99% (HPLC)
Molecular Weight:	250.17

View Full Technical Details for 1400W dihydrochloride

Technical Data
Citations / Reviews (3)

Biological Activity for 1400W dihydrochloride

1400W dihydrochloride is a slow, tight binding, potent and highly selective inhibitor of inducible nitric oxide synthase (K_d = 7 nM). Selective over nNOS and eNOS (K_i values are 2 and 50 μM respectively). Cell-permeable and active in vivo. Neuroprotective in epilepsy models and has analgesic effects in models of mechanical and heat hypersensitivity.

Technical Data for 1400W dihydrochloride

M. Wt	250.17
Formula	C₁₀H₁₅N₃.2HCl
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	214358-33-5
PubChem ID	2733515
InChi Key	WDJHSQZCZGPGAA-UHFFFAOYSA-N
Smiles	Cl.Cl.CC(=N)NCC1=CC=CC(CN)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 1400W dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	25.02	100

Preparing Stock Solutions for 1400W dihydrochloride

The following data is based on the product molecular weight 250.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.00 mL	19.99 mL	39.97 mL
5 mM	0.80 mL	4.00 mL	7.99 mL
10 mM	0.40 mL	2.00 mL	4.00 mL
50 mM	0.08 mL	0.40 mL	0.80 mL

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References for 1400W dihydrochloride

References are publications that support the biological activity of the product.

Putra Inducible nitric oxide synthase inhibitor, 1400W, mitigates DFP-induced long-term neurotoxicity in the rat model. Neurobiol.Dis. 2020 PMID: 30940499
Staunton Inducible nitric oxide synthase inhibition by 1400W limits pain hypersensitivity in a neuropathic pain rat model. Exp.Physiol 2018 PMID: 29441689
Parmentier Selective inhibition of inducible nitric oxide synthase prevents ischaemic brain injury. Br.J.Pharmacol. 1999 PMID: 10385257
Thomsen Selective inhibition of inducible nitric oxide inhibits tumor growth in vivo: studies with 1400W, a novel inhibitor. Cancer Res. 1997 PMID: 9242464
Garvey 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J.Biol.Chem. 1997 PMID: 9030556