3-Deazaneplanocin A hydrochloride

Name: 3-Deazaneplanocin A hydrochloride
Brand: Tocris Bioscience
SKU: 4703

Tocris Bioscience, part of Bio-Techne | Catalog # 4703

Citations (8)

COA / SDS

EZH2 histone methyltransferase inhibitor

Key Product Details

Chemical Name:	(1S,2R,5R)-5-(4-Amino-1H-imidazo[4,5-c]pyridin-1-yl)-3-(hydroxymethyl)-3-cyclopentene-1,2-diol hydrochloride
Purity:	≥98%

View Full Technical Details for 3-Deazaneplanocin A hydrochloride

Technical Data
Citations

Biological Activity for 3-Deazaneplanocin A hydrochloride

3-Deazaneplanocin A hydrochloride is a histone methyltransferase inhibitor; decreases global histone methylation. 3-Deazaneplanocin A hydrochloride inhibits EZH2 histone methyltransferase and s-adenosylhomocysteine (SAH) hydrolase activity. Blocks trimethylation of lysine 27 on histone H3 and lysine 20 on histone H4 in vitro. 3-Deazaneplanocin A hydrochloride induces apoptosis in multiple cancer cell lines and has no apoptotic effect on normal cells. Enhances Oct4 expression in chemically induced pluripotent stem cells (CiPSCs).

DZNep synthesized to Ancillary Material Grade also available.

For more information about how 3-Deazaneplanocin A hydrochloride may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs

Technical Data for 3-Deazaneplanocin A hydrochloride

Formula	C₁₂H₁₄N₄O₃.HCl
Storage	Store at -20°C
Purity	≥98%
CAS Number	120964-45-6

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3-Deazaneplanocin A hydrochloride

Solubility

Soluble Soluble to 10 mM in water

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References for 3-Deazaneplanocin A hydrochloride

References are publications that support the biological activity of the product.

Tan J, Yang X, Zhuang L et al. Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells. Genes Dev. 2007-01-01 PMID: 17437993
Miranda et al. DZNep is a global histone methylation inhibitor that reactivates developmental genes not silenced by DNA methylation. Mol.Cancer Ther., 2009;8:1579 PMID: 19509260
Tseng et al. Synthesis of 3-deazaneplanocin A, a powerful inhibitor of S-adenosylhomocysteine hydrolase with potent and selective in vitro and in vivo antiviral activities. J.Med.Chem., 1989;32:1442 PMID: 2544721
Hou et al. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science, 2013;341:651 PMID: 23868920

3-Deazaneplanocin A hydrochloride

Tocris Bioscience, part of Bio-Techne | Catalog # 4703

Key Product Details

Biological Activity for 3-Deazaneplanocin A hydrochloride

Technical Data for 3-Deazaneplanocin A hydrochloride

Solubility for 3-Deazaneplanocin A hydrochloride

Dilution Calculator

Reconstitution Calculator

References for 3-Deazaneplanocin A hydrochloride

Product Documents for 3-Deazaneplanocin A hydrochloride

COA

Certificate of Analysis

SDS

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