5-Azacytidine

Name: 5-Azacytidine
Brand: Tocris Bioscience
SKU: 3842

Tocris Bioscience, part of Bio-Techne | Catalog # 3842

Citations (10)

COA / SDS

DNA methyltransferase inhibitor

Key Product Details

Description:	DNA methyltransferase inhibitor
Chemical Name:	4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one
Purity:	≥98% (HPLC)
Molecular Weight:	244.2

View Full Technical Details for 5-Azacytidine

Technical Data
Citations

Biological Activity for 5-Azacytidine

5-Azacytidine is a DNA methyltransferase inhibitor. Incorporates into DNA forming covalent adducts with cellular DNMT1, depleting enzyme activity. Induces demethylation and reactivation of silenced genes. Improves the efficiency of reprogramming of stem cells; induces differentiation of mesenchymal stem cells into cardiomyocytes.

Compound Libraries for 5-Azacytidine

5-Azacytidine is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 5-Azacytidine

M. Wt	244.2
Formula	C₈H₁₂N₄O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	320-67-2
PubChem ID	9444
InChi Key	NMUSYJAQQFHJEW-KVTDHHQDSA-N
Smiles	O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N2C(N=C(N)N=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-Azacytidine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	12.21	50
	DMSO	24.42	100

Preparing Stock Solutions for 5-Azacytidine

The following data is based on the product molecular weight 244.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.10 mL	20.48 mL	40.95 mL
5 mM	0.82 mL	4.10 mL	8.19 mL
10 mM	0.41 mL	2.05 mL	4.10 mL
50 mM	0.08 mL	0.41 mL	0.82 mL

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Reconstitution Calculator

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References for 5-Azacytidine

References are publications that support the biological activity of the product.

Mikkelsen Dissecting direct reprogramming through integrative genomic analysis. Nature 2008 PMID: 18509334
Qian 5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase. Stem Cells Dev. 2012 PMID: 21476855
Schneider-Stock 5-aza-Cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. J.Pharmacol.Exp.Ther. 2005 PMID: 15547111

Product Documents for 5-Azacytidine

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Azacitidine, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov