5-BrdU

Name: 5-BrdU
Brand: Tocris Bioscience
SKU: 5015
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5015

(1)

Citations (4)

COA / SDS

Synthetic thymidine analog; used for cell proliferation assays

Key Product Details

Description:	Synthetic thymidine analog; used for cell proliferation assays
Chemical Name:	5-Bromo-2-deoxyuridine
Purity:	≥99% (HPLC)
Molecular Weight:	307.1

View Full Technical Details for 5-BrdU

Technical Data
Citations / Reviews (1)

Biological Activity for 5-BrdU

5-BrdU is a synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.

Compound Libraries for 5-BrdU

5-BrdU is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 5-BrdU

M. Wt	307.1
Formula	C₉H₁₁BrN₂O₅
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	59-14-3
PubChem ID	6035
InChi Key	WOVKYSAHUYNSMH-RRKCRQDMSA-N
Smiles	O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-BrdU

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	15.36	50
	DMSO	30.71	100

Preparing Stock Solutions for 5-BrdU

The following data is based on the product molecular weight 307.1.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.26 mL	16.28 mL	32.56 mL
5 mM	0.65 mL	3.26 mL	6.51 mL
10 mM	0.33 mL	1.63 mL	3.26 mL
50 mM	0.07 mL	0.33 mL	0.65 mL

Molarity Calculator

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Reconstitution Calculator

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References for 5-BrdU

References are publications that support the biological activity of the product.

Barker A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary. PLoS One 2013 PMID: 23691086
Long Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells. Cell Res. 2015 PMID: 26251165
Stacey and Hitomi Cell cycle studies basd upon quantitative image analysis. Cytometry A 2008 PMID: 18163464