5-Fluorouracil

Name: 5-Fluorouracil
Brand: Tocris Bioscience
SKU: 3257

Tocris Bioscience, part of Bio-Techne | Catalog # 3257

Citations (26)

COA / SDS

Thymidylate synthetase inhibitor

Key Product Details

Description:	Thymidylate synthetase inhibitor
Alternative Names:	5-FU
Chemical Name:	5-Fluoro-2,4-(1H,3H)-pyrimidinedione
Purity:	≥99% (HPLC)
Molecular Weight:	130.08

View Full Technical Details for 5-Fluorouracil

Technical Data
Citations

Biological Activity for 5-Fluorouracil

5-Fluorouracil is an anticancer agent. Metabolized to form fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine (FUTP). FdUMP inhibits thymidylate synthase, causing a reduction in dTMP synthesis. FUTP and FdUTP are misincorporated into RNA and DNA respectively.

Technical Data for 5-Fluorouracil

M. Wt	130.08
Formula	C₄H₃FN₂O₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	51-21-8
PubChem ID	3385
InChi Key	GHASVSINZRGABV-UHFFFAOYSA-N
Smiles	O=C1C(F)=CNC(N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-Fluorouracil

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	13.01	100
	ethanol	1.3	10

Preparing Stock Solutions for 5-Fluorouracil

The following data is based on the product molecular weight 130.08.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	7.69 mL	38.44 mL	76.88 mL
5 mM	1.54 mL	7.69 mL	15.38 mL
10 mM	0.77 mL	3.84 mL	7.69 mL
50 mM	0.15 mL	0.77 mL	1.54 mL

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Reconstitution Calculator

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References for 5-Fluorouracil

References are publications that support the biological activity of the product.

Peters Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim.Biophys.Acta. 2002 PMID: 12084461
Longley 5-Fluorouracil: mechanisms of action and clinical strategies. Nat.Rev.Cancer 2003 PMID: 12724731
Ghoshal and Jacob An alternative molecular mechanism of action of 5-fluorouracil, a potent anticancer drug. Biochem.Pharmacol. 1997 PMID: 9264308

Product Documents for 5-Fluorouracil

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Fluorouracil, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov