A 196

Name: A 196
Brand: Tocris Bioscience
SKU: 6167

Tocris Bioscience, part of Bio-Techne | Catalog # 6167

Citations (2)

COA / SDS

Potent and selective SUV420H1/H2 inhibitor

Key Product Details

Description:	Potent and selective SUV420H1/H2 inhibitor
Chemical Name:	6,7-Dichloro-N-cyclopentyl-4-(4-pyridinyl)-1-phthalazinamine
Purity:	≥98% (HPLC)
Molecular Weight:	359.25

View Full Technical Details for A 196

Technical Data
Citations

Biological Activity for A 196

A 196 is a potent and selective SUV420H1/H2 inhibitor (IC₅₀ values are 25 and 144 nM respectively) that exhibits 100-fold selectivity over other histone methyltransferases and non-epigenetic targets. A 196 inhibits the di- and tri-methylation of H4K20me in multiple cell lines (IC₅₀ < 1 μM).

To request the negative control for A 196, please fill out the SGC 2043 request form on the SGC website.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the A-196 probe summary on the SGC website.

Compound Libraries for A 196

A 196 is also offered as part of the Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of A196 is reviewed on the Chemical Probes website.

Technical Data for A 196

M. Wt	359.25
Formula	C₁₈H₁₆Cl₂N₄
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1982372-88-2
PubChem ID	117072548
InChi Key	ABGOSOMRWSYAOB-UHFFFAOYSA-N
Smiles	ClC1=CC2=C(C(C3=CC=NC=C3)=NN=C2NC4CCCC4)C=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for A 196

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.18	20

Preparing Stock Solutions for A 196

The following data is based on the product molecular weight 359.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	13.92 mL	69.59 mL	139.18 mL
1 mM	2.78 mL	13.92 mL	27.84 mL
2 mM	1.39 mL	6.96 mL	13.92 mL
10 mM	0.28 mL	1.39 mL	2.78 mL

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References for A 196

References are publications that support the biological activity of the product.

Scheer A chemical biology toolbox to study protein methyltransferases and epigenetic signaling. Nat.Commun. 2019 PMID: 30604761
Brustel Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication. EMBO J 2017 PMID: 28778956
Bromberg The SUV4-20 inhibitor A-196 verifies a role for epigenetics in genomic integrity. Nat.Chem.Biol. 2017 PMID: 28114273