Actinomycin D

Name: Actinomycin D
Brand: Tocris Bioscience
SKU: 1229
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1229

(2)

Citations (93)

COA / SDS

Inhibits RNA polymerase

Key Product Details

Description:	Inhibits RNA polymerase
Chemical Name:	2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Purity:	≥95% (HPLC)
Molecular Weight:	1255.43

View Full Technical Details for Actinomycin D

Technical Data
Citations / Reviews (2)

Biological Activity for Actinomycin D

Actinomycin D is an anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.

Technical Data for Actinomycin D

M. Wt	1255.43
Formula	C₆₂H₈₆N₁₂O₁₆
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	50-76-0
PubChem ID	2019
InChi Key	RJURFGZVJUQBHK-DCUCITLNSA-N
Smiles	[H][C@@]12CCCN1C([C@H](C(C)C)NC([C@H]([C@H](OC([C@@H](N(C(CN(C2=O)C)=O)C)C(C)C)=O)C)NC(C3=C4N=C5C(OC4=C(C=C3)C)=C(C(C(N)=C5C(N[C@H]6[C@H](OC([C@@H](N(C(CN(C([C@@]7(CCCN7C([C@H](C(C)C)NC6=O)=O)[H])=O)C)=O)C)C(C)C)=O)C)=O)=O)C)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Actinomycin D

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	62.77	50

Preparing Stock Solutions for Actinomycin D

The following data is based on the product molecular weight 1255.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	1.59 mL	7.97 mL	15.93 mL
2.5 mM	0.32 mL	1.59 mL	3.19 mL
5 mM	0.16 mL	0.80 mL	1.59 mL
25 mM	0.03 mL	0.16 mL	0.32 mL

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Reconstitution Calculator

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References for Actinomycin D

References are publications that support the biological activity of the product.

Glynn Apoptosis induced by actinomycin D, camptothecin or aphidicolin can occur in all phases of the cell cycle. Biochem.Soc.Trans. 1992 PMID: 1634006
Jeeninga The mechanism of actinomycin D-mediated inhibition of HIV-1 reverse transcription. Nucleic Acids Res. 1998 PMID: 9826774
Aktipis A kinetic study on the mechanism of inhibition of RNA synthesis catalyzed by DNA-dependent RNA polymerase. Differences in inhibition by ethidium bromide, 3,8-diamino-6-ethylphenanthridinium bromide and actinomycin D. Biochim.Biophys.Acta 1981 PMID: 7025910

Product Documents for Actinomycin D

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Actinomycin D, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov