Akti-1/2

Name: Akti-1/2
Brand: Tocris Bioscience
SKU: 5773
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5773

(2)

Citations (20)

COA / SDS

Potent and selective dual Akt1 and 2 inhibitor

Key Product Details

Description:	Potent and selective dual Akt1 and 2 inhibitor
Alternative Names:	Akt Inhibitor VIII
Chemical Name:	1,3-Dihydro-1-[1-[[4-(6-phenyl-1H-imidazo[4,5-g]quinoxalin-7-yl)phenyl]methyl]-4-piperidinyl]-2H-benzimidazol-2-one
Purity:	≥98% (HPLC)
Molecular Weight:	551.64

View Full Technical Details for Akti-1/2

Technical Data
Citations / Reviews (2)

Biological Activity for Akti-1/2

Akti-1/2 is a potent and selective dual Akt1 and 2 inhibitor (IC₅₀ values are 50 and 210 nM, respectively). Selective for Akt1 and 2 over a panel of other tyrosine and serine/threonine kinases. Sensitizes LnCaP cells to TRAIL (TNF-related apoptosis-inducing ligand) induced apoptosis. Also enhances CAR and TCR retroviral transduction of human T cells. Active in vivo.

Technical Data for Akti-1/2

M. Wt	551.64
Formula	C₃₄H₂₉N₇O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	612847-09-3
PubChem ID	10196499
InChi Key	BIWGYFZAEWGBAL-UHFFFAOYSA-N
Smiles	O=C1NC2=C(C=CC=C2)N1C(CC3)CCN3CC(C=C4)=CC=C4C5=NC6=CC(N=CN7)=C7C=C6N=C5C8=CC=CC=C8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Akti-1/2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	11.03	20 with gentle warming

Preparing Stock Solutions for Akti-1/2

The following data is based on the product molecular weight 551.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	9.06 mL	45.32 mL	90.64 mL
1 mM	1.81 mL	9.06 mL	18.13 mL
2 mM	0.91 mL	4.53 mL	9.06 mL
10 mM	0.18 mL	0.91 mL	1.81 mL

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References for Akti-1/2

References are publications that support the biological activity of the product.

Klebanoff Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy. JCI Insight 2017 PMID: 29212954
Bain The selectivity of protein kinase inhibitors: a further update. Biochem.J. 2007 PMID: 17850214
Barnett Identification and characterization of pleckstrin-homology-domain-dependent and isoenzyme-specific Akt inhibitors. Biochem. J. 2005 PMID: 15456405
Lindsley Allosteric Akt (PKB) inhibitors: discovery and SAR of isozyme selective inhibitors. Bioorg.Med.Chem.Lett. 2005 PMID: 15664853
DeFeo-Jones D Tumor cell sensitization to apoptotic stimuli by selective inhibition of specific Akt/PKB family members. Mol.Cancer Ther. 2005 PMID: 15713898