AZ1 USP25/28 inhibitor

Name: AZ1 USP25/28 inhibitor
Brand: Tocris Bioscience
SKU: 7845

Tocris Bioscience, part of Bio-Techne | Catalog # 7845

Citations (1)

COA / SDS

Selective USP25 and USP28 inhibitor

Key Product Details

Description:	Selective USP25 and USP28 inhibitor
Chemical Name:	2-[[[5-Bromo-2-[[4-fluoro-3-(trifluoromethyl)phenyl]methoxy]phenyl]methyl]amino]ethanol
Purity:	≥98% (HPLC)
Molecular Weight:	422.21

View Full Technical Details for AZ1 USP25/28 inhibitor

Technical Data
Citations

Biological Activity for AZ1 USP25/28 inhibitor

AZ1 USP25/28 inhibitor is a selective inhibitor of USP28 (IC₅₀ = 0.6 μM, K_d= 0.2 - 3.7 μM) and USP25 (IC₅₀ = 0.7 μM). AZ1 USP25/28 inhibitor exhibits selectivity for USP25 and USP28 over other DUB family members. The compound dose-dependently reduces c-Myc levels in colon carcinoma cells in vitro and induces cell death (EC₅₀ values are 18 - 20 μM). In a mouse Alzheimer's disease model, AZ1 USP25/28 inhibitor reduces amyloid burden, attenuates microglial activation and improves synaptic and cognitive function.

Technical Data for AZ1 USP25/28 inhibitor

M. Wt	422.21
Formula	C₁₇H₁₆BrF₄NO₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2165322-94-9
PubChem ID	135397656
InChi Key	ITHSFXDGKQYOED-UHFFFAOYSA-N
Smiles	FC1=CC=C(COC2=CC=C(C=C2CNCCO)Br)C=C1C(F)(F)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AZ1 USP25/28 inhibitor

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.22	100

Preparing Stock Solutions for AZ1 USP25/28 inhibitor

The following data is based on the product molecular weight 422.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.37 mL	11.84 mL	23.68 mL
5 mM	0.47 mL	2.37 mL	4.74 mL
10 mM	0.24 mL	1.18 mL	2.37 mL
50 mM	0.05 mL	0.24 mL	0.47 mL

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References for AZ1 USP25/28 inhibitor

References are publications that support the biological activity of the product.

Zheng Trisomy 21-induced dysregulation of microglial homeostasis in Alzheimer's brains is mediated by USP25. Sci.Adv. 2021 PMID: 33523861
Zheng USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generation. J.Clin.Invest. 2022 PMID: 35229730
Wrigley Identification and characterization of dual inhibitors of the USP25/28 deubiquitinating enzyme subfamily. ACS Chem.Biol. 2017 PMID: 29131570