B 973B

Name: B 973B
Brand: Tocris Bioscience
SKU: 6764

Tocris Bioscience, part of Bio-Techne | Catalog # 6764

COA / SDS

Positive allosteric modulator of α7 nAChRs; analgesic

Key Product Details

Description:	Positive allosteric modulator of α7 nAChRs; analgesic
Alternative Names:	(-)-(S)-B 973B
Chemical Name:	3,4-Difluoro-N-[(1S)-1-[6-[4-(2-pyridinyl)-1-piperazinyl]-2-pyrazinyl]ethyl]benzenepropanamide
Purity:	≥98% (HPLC)
Molecular Weight:	452.5

View Full Technical Details for B 973B

Technical Data
Citations

Biological Activity for B 973B

B 973B is a positive allosteric modulator of α7 nAChRs (EC₅₀ ~0.3 μM); active enantiomer. Increases potency of acetylcholine (EC₅₀ values are 0.007 mM and 0.3 mM in the presence and absence of B 937B, respectively). Potentiates peak acetylcholine-induced currents 6-fold relative to maximal acetylcholine, and slows channel desensitization, resulting in a 6900-fold increase in charge transfer. Decreases paw edema in vivo. Analgesic.

Compound Libraries for B 973B

B 973B is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for B 973B

M. Wt	452.5
Formula	C₂₄H₂₆F₂N₆O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2244989-34-0
PubChem ID	137319851
InChi Key	WQYZERNSONBUCW-KRWDZBQOSA-N
Smiles	C[C@@H](C1=CN=CC(N2CCN(C3=CC=CC=N3)CC2)=N1)NC(CCC4=CC(F)=C(C=C4)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for B 973B

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	45.25	100
	ethanol	22.62	50

Preparing Stock Solutions for B 973B

The following data is based on the product molecular weight 452.5.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.21 mL	11.05 mL	22.10 mL
5 mM	0.44 mL	2.21 mL	4.42 mL
10 mM	0.22 mL	1.10 mL	2.21 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for B 973B

References are publications that support the biological activity of the product.

Post-Munson B-973, a novel piperazine positive allosteric modulator of the α7 nicotinic acetylcholine receptor. Eur.J.Pharmacol. 2017 PMID: 28132910
Quadri Macroscopic and microscopic activation of a7 nicotinic acetylcholine receptors by the structurally unrelated allosteric agonist-positive allosteric modulators (ago-PAMs) B-973B and GAT107. Mol.Pharmacol. 2019 PMID: 30348894
Garai B-973, a novel α7 nAChR Ago-PAM: racemic and asymmetric synthesis, electrophysiological studies, and in vivo evaluation. ACS Med.Chem.Lett. 2018 PMID: 30429960