BC-LI-0186

Name: BC-LI-0186
Brand: Tocris Bioscience
SKU: 6791

Tocris Bioscience, part of Bio-Techne | Catalog # 6791

Citations (1)

COA / SDS

Leucyl-tRNA synthase (TRS)/Ras-related GTP-binding protein D (RagD) interaction inhibitor

Key Product Details

Description:	Leucyl-tRNA synthase (TRS)/Ras-related GTP-binding protein D (RagD) interaction inhibitor
Chemical Name:	4-[2,5-Dihydro-2,3-dimethyl-4-(1-methylethyl)-5-oxo-1H-pyrazol-1-yl]-N-(2-phenoxyethyl)benzenesulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	429.54

View Full Technical Details for BC-LI-0186

Technical Data
Citations

Biological Activity for BC-LI-0186

BC-LI-0186 is a potent and high affinity leucyl-tRNA synthase (TRS)/Ras-related GTP-binding protein D (RagD) interaction inhibitor (IC₅₀ = 46.1 nM; K_d = 42.1 nM for binding to LRS). Inhibits mTOR complex 1 activity by blocking binding to LRS without affecting catalytic activity. Inhibits leucine-induced translocation of LRS to the lysosome.

Compound Libraries for BC-LI-0186

BC-LI-0186 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for BC-LI-0186

M. Wt	429.54
Formula	C₂₂H₂₇N₃O₄S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	695207-56-8
PubChem ID	4917788
InChi Key	SQYWMHPZMHDCHP-UHFFFAOYSA-N
Smiles	CC(C1=C(N(N(C2=CC=C(S(=O)(NCCOC3=CC=CC=C3)=O)C=C2)C1=O)C)C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BC-LI-0186

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.95	100

Preparing Stock Solutions for BC-LI-0186

The following data is based on the product molecular weight 429.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.33 mL	11.64 mL	23.28 mL
5 mM	0.47 mL	2.33 mL	4.66 mL
10 mM	0.23 mL	1.16 mL	2.33 mL
50 mM	0.05 mL	0.23 mL	0.47 mL

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References for BC-LI-0186

References are publications that support the biological activity of the product.

Choi Leucine-induced localization of Leucyl-tRNA synthetase in lysosome membrane. Biochem.Biophys.Res.Commun. 2017 PMID: 28882589
Lee Coordination of the leucine-sensing Rag GTPase cycle by leucyl-tRNA synthetase in the mTORC1 signaling pathway. Proc.Natl.Acad.Sci.USA. 2018 PMID: 29784813
Kim Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction. Nat.Commun. 2017 PMID: 28963468