BIRB 796

Name: BIRB 796
Brand: Tocris Bioscience
SKU: 5989

Tocris Bioscience, part of Bio-Techne | Catalog # 5989

Citations (36)

COA / SDS

High affinity and selective p38 kinase inhibitor

Key Product Details

Description:	High affinity and selective p38 kinase inhibitor
Alternative Names:	Doramapimod
Chemical Name:	N-[3-(1,1-Dimethylethyl)-1-(4-methylphenyl)-1H-pyrazol-5-yl]-N'-[4-[2-(4-morpholinyl)ethoxy]-1-naphthalenyl]urea
Purity:	≥98% (HPLC)
Molecular Weight:	527.66

View Full Technical Details for BIRB 796

Technical Data
Citations

Biological Activity for BIRB 796

BIRB 796 is a high affinity and selective p38 kinase inhibitor (K_d = 50-100 pM). Exhibits no significant inhibition on a panel of related kinases. Inhibits LPS-induced TNFα production in human PBMCs and whole blood (IC₅₀ values are 21 and 960 nM, respectively). Also inhibits JNK2α2 and c-Raf-1 (IC₅₀ values are 98 nM and 1.4 μM, respectively). BIRB 796 can be used in protocols for the chemical reprogramming of somatic cells to iPSCs. Cell permeable.

Compound Libraries for BIRB 796

BIRB 796 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for BIRB 796

M. Wt	527.66
Formula	C₃₁H₃₇N₅O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	285983-48-4
PubChem ID	156422
InChi Key	MVCOAUNKQVWQHZ-UHFFFAOYSA-N
Smiles	CC1=CC=C(N2N=C(C(C)(C)C)C=C2NC(NC3=CC=C(C4=C3C=CC=C4)OCCN5CCOCC5)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BIRB 796

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	5.28	10

Preparing Stock Solutions for BIRB 796

The following data is based on the product molecular weight 527.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	18.95 mL	94.76 mL	189.52 mL
0.5 mM	3.79 mL	18.95 mL	37.90 mL
1 mM	1.90 mL	9.48 mL	18.95 mL
5 mM	0.38 mL	1.90 mL	3.79 mL

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References for BIRB 796

References are publications that support the biological activity of the product.

Guan Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 2022 PMID: 35418683
Kuma BIRB796 inhibits all p38 MAPK isoforms in vitro and in vivo. J.Biol.Chem. 2005 PMID: 15755732
Regan Structure-activity relationships of the p38alpha MAP kinase inhibitor 1-(5-tert-butyl-2-p-tolyl-2H-pyrazol-3-yl)-3-[4-(2-morpholin-4-yl-ethoxy)naph- thalen-1-yl]urea (BIRB 796). J.Med.Chem. 2003 PMID: 14561087
Goldstein Selective p38α inhibitors clinically evaluated for the treatment of chronic inflammatory disorders. J.Med.Chem. 2010 PMID: 19950901
Laufer Design, synthesis, and biological evaluation of novel Tri- and tetrasubstituted imidazoles as highly potent and specific ATP-mimetic inhibitors of p38 MAP kinase: focus on optimized interactions with the enzyme's surface-exposed front region. J.Med.Chem. 2008 PMID: 18578517