Brefeldin A

Name: Brefeldin A
Brand: Tocris Bioscience
SKU: 1231
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1231

(1)

Citations (77)

COA / SDS

Disrupts protein translocation to Golgi

Key Product Details

Description:	Disrupts protein translocation to Golgi
Chemical Name:	1,6,7,8,9,11aβ,12,13,14,14aα-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent[f]oxacyclotridecin-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	280.36

View Full Technical Details for Brefeldin A

Technical Data
Citations / Reviews (1)

Biological Activity for Brefeldin A

Brefeldin A is a reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor (ARF1) to the Golgi apparatus and inhibits GDP-GTP exchange, leading to activation of ER stress signaling pathways. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). Antifungal.

Technical Data for Brefeldin A

M. Wt	280.36
Formula	C₁₆H₂₄O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	20350-15-6
PubChem ID	6436187
InChi Key	KQNZDYYTLMIZCT-SZXCEISTSA-N
Smiles	O[C@@H]([C@H]1[C@H](/C=C/CCC[C@H](C)O2)C[C@H](O)C1)/C=C/C2=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Brefeldin A

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	14.02	50

Preparing Stock Solutions for Brefeldin A

The following data is based on the product molecular weight 280.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	7.13 mL	35.67 mL	71.34 mL
2.5 mM	1.43 mL	7.13 mL	14.27 mL
5 mM	0.71 mL	3.57 mL	7.13 mL
25 mM	0.14 mL	0.71 mL	1.43 mL

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References for Brefeldin A

References are publications that support the biological activity of the product.

Oh-Hashi Elucidation of brefeldin A-induced ER and Golgi stress responses in Neuro2a cells. Mol. Cell. Biochem. 2021 PMID: 34129155
Yu Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 2015 PMID: 25658371
Ding Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 2007 PMID: 17135238
Tsai Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 1994 PMID: 8159707
Morinaga Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8917509
Ktistakis Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 1992 PMID: 1549178