Bumetanide

Name: Bumetanide
Brand: Tocris Bioscience
SKU: 3108

Tocris Bioscience, part of Bio-Techne | Catalog # 3108

Citations (78)

COA / SDS

Na+/2Cl-/K+ (NKCC) symporter inhibitor

Key Product Details

Description:	Na⁺/2Cl^-/K⁺ (NKCC) symporter inhibitor
Chemical Name:	3-(Aminosulfonyl)-5-(butylamino)-4-phenoxybenzoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	364.42

View Full Technical Details for Bumetanide

Technical Data
Citations

Biological Activity for Bumetanide

Bumetanide is a loop diuretic that inhibits the Na⁺/2Cl^-/K⁺ (NKCC) cotransporter. More potent than furosemide (Cat. No. 3109). Bumetanide improves electrophysiological, pathological and cognitive deficits in APOE4 knock-in mice. RNA sequencing of neurons derived from APOE4 induced pluripotent stem cell (iPSC) shows Bumetanide reverses Alzheimer's disease transcriptomic signatures.

Compound Libraries for Bumetanide

Bumetanide is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Bumetanide

M. Wt	364.42
Formula	C₁₇H₂₀N₂O₅S
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	28395-03-1
PubChem ID	2471
InChi Key	MAEIEVLCKWDQJH-UHFFFAOYSA-N
Smiles	CCCCNC1=CC(C(O)=O)=CC(S(=O)(N)=O)=C1OC2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Bumetanide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36.44	100

Preparing Stock Solutions for Bumetanide

The following data is based on the product molecular weight 364.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.74 mL	13.72 mL	27.44 mL
5 mM	0.55 mL	2.74 mL	5.49 mL
10 mM	0.27 mL	1.37 mL	2.74 mL
50 mM	0.05 mL	0.27 mL	0.55 mL

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Reconstitution Calculator

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References for Bumetanide

References are publications that support the biological activity of the product.

Taubes Experimental and real-world evidence supporting the computational repurposing of bumetanide for APOE4-related Alzheimer's disease. Nature Aging 2021 PMID: 36172600
Isenring and Forbush Ion and bumet. binding by the Na-K-Cl cotransporter. J.Biol.Chem. 1997 PMID: 9305921
Morita Functional evidence for involvement of bumetanide-sensitive Na⁺K⁺2Cl^- cotransport in the hepatoportal Na⁺ receptor of the sprague-dawley rat. Neurosci.Letts. 1999 PMID: 10320015
O'Grady Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am.J.Physiol. 1987 PMID: 3303961